Reagents for determining peptidoglycan and .beta.-1,3-glucan
AssigneeWako Pure Chemical Industries, Ltd.
InventorsMasaaki Ashida, Masakazu Tsuchiya, Yoshitsugu Sakata, Shuji Matsuura
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Abstract
A reagent comprising a fraction obtained from plasma of an insect such as silkworm larvae and capable of reacting specifically with .beta.-1,3-glucan or peptidoglycan can be used for determining .beta.-1,3-glucan or peptidoglycan.
Claims12
A partially purified reagent for determining .beta.-1,3-glucan comprising a fraction prepared by the process of treating plasma of an insect with a method selected from the group consisting of gel filtration, electrophoresis, high performance liquid chromatography and affinity chromatography, wherein a substance which reacts with peptidoglycan is removed from said plasma, and said fraction is capable of reacting specifically with .beta.-1,3-glucan in the presence of a zymogen of an esterase hydrolyzing N-.alpha.-benzoyl-L-arginine ethyl ester, prophenoloxidase activating enzyme or phenoloxidase to activate said zymogen.
A partially purified reagent for determining peptidoglycan comprising a fraction prepared by the process of treating insect plasma with a method selected from the group consisting of gel filtration, electrophoresis, high performance liquid chromatography and affinity chromatography, wherein a substance which reacts with .beta.1,3-glucan is removed from said plasma, and said fraction is capable of reacting specifically with peptidoglycan in the presence of a zymogen of an esterase hydrolyzing N-.alpha.-benzoyl-L-arginine ethyl ester, prophenoloxidase activating enzyme or phenoloxidase to activate said zymogen.
Dependencies are inferred from the claim text.