Method for rapid base sequencing in DNA and RNA

A method for base sequencing of DNA or RNA fragments, comprising the steps of:

• forming said fragments with bases having identifiable characteristics;
• sequentially cleaving single identifiable bases from a single one of said fragments to form a train of said identifiable bases;
• and
• identifying said single, cleaved bases in said train.

A method for DNA or RNA base sequencing, comprising the steps of;

• modifying each nucleotide effective for DNA or RNA synthesis to attach a fluorescent dye characteristic of that nucleotide with a linker arm effective to enable DNA or RNA synthesis and exonuclease cleavage;
• synthesizing from said modified nucleotides a strand of DNA complementary to a DNA or RNA strand having a base sequence to be determined;
• cleaving each said modified nucleotide sequentially from a single fragment containing said complementary DNA strand;
• and
• fluorescing each said characteristic dye to identify said sequence of nucleotides.