Plant promotors
AssigneeDNA Plant Technology Corporation
InventorsJohn R. Bedbrook, Caroline Dean, Pamela Dunsmuir
Life of this patent
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Abstract
Promoter sequences from the gene from the small subunit of ribulose-1,5-bisphosphate carboxylase are disclosed. Expression cassettes containing a promoter sequence, a linker region, and a 3' fragment are also disclosed. The promotor sequences and expression cassettes are useful for expressing foreign genes to high levels in transformed plants.
Claims14
An isolated recombinant DNA molecule comprising a DNA promoter sequence capable of directing transcription of downstream foreign structural genes in plants, said sequence comprising less than about 5,000 base pairs of 5' region DNA counted upstream from the translation start site of the Petunia gene for the small subunit ribulose-1, 5-bisphosphate carboxylase and said sequence selected from the group consisting of SSU301 and SSU611.
An expression cassette for use in expressing foreign structural genes in plants, said expression cassette comprising
- (a) promoter DNA comprising less than about 5,000 base pairs of 5' region DNA counted upstream from the translation start site of the Petunia gene for the small subunit of ribulose-1, 5-bisphosphate carboxylase, said promoter DNA being modified to create a first restriction site at the 3' end of the 5' region DNA and said promoter DNA selected from the group consisting of SSU301 and SSU611;
- (b) linker DNA fused to said first restriction site;
- and
- (c) 3' region DNA comprising DNA from the 3' region of a eukaryotic gene, said 3' region DNA having a second restriction site at its 5' end and said 3' region DNA being fused to said linker DNA at said second restriction site.
An expression cassette comprising less than about 5,000 base pairs of 5' region DNA counted upstream from the translation start site of the small subunit of Petunia ribulose-1, 5-bisphosphate carboxylase, wherein said 5' region DNA is selected from the group consisting of SSU301 and SSU611.
Bacterial strain ATCC #67125.
Dependencies are inferred from the claim text.