Fermentation process to produce 2-keto-L-gluonic acid
AssigneeHoffmann-La Roche Inc.
InventorsTatsuo Hoshino, Setsuko Nomura, Teruhide Sugisawa
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Abstract
A process of producing 2-keto-L-gulonic acid by fermentative conversion of L-sorbose utilizing a fermentation system composed of component produced from a microorganism having the identifying characteristics of strain DSM No. 4025 and a yeast component.
Claims30
A process for producing 2-keto-L-gluconic acid comprising fermentatively converting in a nutrient medium, L-sorbose to 2-keto-L-gluconic acid by the action of fermentation system containing:
- a. a biologically homogeneous culture of a microorganism having the identifying characteristics of strain DSM No. 4025, and having substantially no ability to produce 2-keto-L-gluconic acid from L-sorbose;
- and
- b. a yeast or a component obtained from said yeast having the growth promoting activity of the yeast from which said component is obtained.
The process of wherein the fermentative conversion is carried out at pH between about 4.0 and 9.0.
A process of producing 2-keto-L-gluconic acid comprising fermentatively converting, in a nutrient medium, L-sorbose to 2keto-L-gluconic acid by the action of a fermentation system containing:
- a. a cell free or whole cell extract obtained from a biologically homogeneous culture of microorganism having the identifying characteristics of strain DSM No. 4025, and having substantially no ability to produce 2-keto-gluonic acid from L-sorbose;
- and
- b. a yeast or component obtained therefrom having the growth promoting activity of the yeast from which said component is obtained.
The process of claim wherein said yeast belongs to the genus Schizosaccharomyces.
The process of claim 25 wherein the yeast is Pichia membranaefaciens or Hansenula anomala.
Dependencies are inferred from the claim text.