US 4,959,211 AGranted

Process for the production on an antiviral vaccine, particularly anti-foot and mouth disease vaccine

OwnerJorge H. Lombardo, Eduardo E. Smolko +2inventors

InventorsJorge H. Lombardo, Eduardo E. Smolko, Rodolfo A. Ugalde, Scholein Rivenson

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Abstract

The instant invention relates to a process in which important improvements are introduced in virus production and inactivation steps, as well as in the vaccine formulation step. The virus is reproduced in animals immuno-depressed through ionizing radiations, thus making it possible to use adult host animals, not naturally sensitive to the virus, with the advantage of using all types of tissues thereof. Bacterial infections are prevented by antibiotic administration. Virus inactivation is also obtained through ionizing radiations. Radiation doses do not affect antigenic properties, as may be noted from the interpretation of the Target Theory.

Claims17

Dependent claims

A process for preparing an antiviral viccine, paticularly anti-foot and mouth disease vaccine, which process comprises the steps of:

(a) radiating an animal free of active virus with ionizing radiations;
(b) innoculating active virus into said animal;
(c) maintaining the animal under controlled sterile conditions and under antibiotic administration until a high viral proliferation is obtained;
(d) after animal's death, cooling the cadaver;
(e) withdrawing skin and viscerae from the animal cadaver;
(f) crushing the soft portions of the remaining parts of the cadaver with the aid of a saline solution in order to obtain a visually homogeneous mixture;
(g) mechanically separating an active viral suspension from said mixture;
(h) transforming the active viral suspension into an inactive viral suspension through the application of ionizing radiation on said suspensions, which are maintained at a temperature substantially lower than 0.degree. C.;
and
(i) formulating the vaccine from said inactive viral suspension.

7 more elements

Dependencies are inferred from the claim text.