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US 4,950,599 AGranted
Method for exchanging homologous DNA sequences in a cell using polyoma encapsulated DNA fragments
OwnerWolf Bertlinginventor
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OwnerWolf Bertlinginventor
Estimating the term…
A method for altering a cell by exchanging a preselected cellular DNA sequence with an exogenous DNA sequence different from the cellular DNA sequence employs an exogenous DNA sequence encapsidated in a polyoma or polyoma-like capsid. The polyoma capsid is then contacted to the cell so that the exogenous DNA sequence is introduced within the cell and exchanges with the preselected cellular DNA sequence by homologous recombination. A preferred article of manufacture comprises a polyoma capsid and a plurality of DNA sequences encapsulated within the polyoma capsid. The DNA sequences each comprise not more than an incomplete portion of a single preselected gene. The exogenous DNA sequence may optionally be complexed to a DNA binding protein, such as a recA protein, prior to encapsulating the exogenous DNA sequence within a viral capsid, so that the uptake of the DNA sequence into the capsid is enhanced.
A method of altering, by homologous recombination, a preselected chromosomal DNA sequence encoding a protein in a polyoma-permissive cell, said method comprising the steps of:
An article of manufacture useful for altering by homologous recombination a preselected chromosomal DNA sequence encoding a protein in a polyoma-permissive cell, said article comprising a polyoma viral capsid and at least one exogenous DNA sequence encapsulated within said polyoma viral capsid, said exogenous DNA sequence being sufficiently homologous to a strand of said preselected chromosomal DNA sequence to anneal to the complement thereof.
An article of manufacture useful for altering by homologous recombination a preselected chromosomal DNA sequence encoding a protein in a polyoma-permissive cell, said article comprising a polyoma capsid and at least one DNA sequence encapsulated within said polyoma capsid, said DNA sequence comprising not more than an incomplete portion of said chromosomal DNA sequence and having a length of from about 100 to about 300 nucleotides.
Dependencies are inferred from the claim text.