Genus-specific listeria antigen identified by monoclonal antibodies
AssigneeAkzo N.V.
InventorsBryan T. Butman, Jerome A. Mattingly
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Abstract
Antigens characteristic of all species of Listeria except L. denitrificans comprising proteins found in Listeria heat extracts, the major antigen having a molecular weight of from about 30 to about 38KD and comprising three immunogenically different epitopes and others comprising proteins having a molecular weight range of approximately 17KD to the major antigen and comprising an epitope immunoreactive with antibodies that are also reactive with one of the three epitopes on the 30 to about 38KD protein. The invention also comprises mouse monoclonal antibodies specifically reactive with the identified epitopes on these antigens.
Claims16
A Listeria antigen comprising a protein found in Listeria heat extracts, said antigen having a molecular weight of from about 30 to about 38 KD, the antigen comprising three immunogenically different epitopes to which antibodies produced by spleen cells of mice immunized with said Listeria heat extracts are specifically reactive, said antigen being found in heat extracts of all species of Listeria except L. dentirificans and not found in Micrococcus varians, Staphylococcus aureus, S. epidermidis, S. haemolyticus, Streptococcus cremoris, S. bovis, S. pyrogenes, S. thermophilus, S. fecalis, Lactobacillus casei, Actinomyces pyogenes, Bacillus cereus, Erysipelothrix rhusiopathiae, Pseudomonas fluorescens, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli or Salmonella typhi.
A Listeria antigen comprising a protein found in Listeria heat extracts, said antigen having a molecular weight less than 30 KD but at least 17 KD, and said antigen containing an epitope which is also present on a 30-30 KD protein antigen found in heat extracts of all species of Listeria except L. denitrificans and not found in Micrococcus varians, Staphylococcus aureus, S. epidermidis, S. haemolyticus, Streptococcus cremoris, S. bovis, S. pyrogenes, S. thermophilus, S. fecalis, Lactobacillus casei, Actinomyces pyrogenes, Bacillus cereus, Erysipelothrix rhusiopathiae, Pseudomonas fluorescens, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli or Salmonella typhi.
Dependencies are inferred from the claim text.