Bacillus brevis strains and application thereof
AssigneeHigeta Shoyu Co., Ltd.
InventorsShigezo Udaka, Hiroaki Takagi, Kiyoshi Kadowaki
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Abstract
Bacillus brevis strains which produce a large amount of protein but no protease out of the cells are disclosed. These strains are highly useful as hosts in genetic engineering.
Claims2
A Bacillus brevis selected from the group consisting of Bacillus brevis H102 (FERM BP-1087) and Bacillus brevis H503 (FERM BP-1088) strain in substantially pure form.
A method of preparing a desired exopeptide or exoprotein by means of recombinant DNA, which comprises using as a host a Bacillus brevis selected from the group consisting of Bacillus brevis H102 (FERM BP-1087) and Bacillus brevis #503 (FERM BP-1088) capable of producing exoprotein but incapable of producing exoprotease which decomposes bovine serum albumin and also incapable of producing exoprotease which decomposes casein.
Dependencies are inferred from the claim text.