Single polypeptide chain binding molecules
AssigneeGenex Corporation
InventorsRobert C. Ladner, Robert E. Bird, Karl Hardman
Life of this patent
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Abstract
The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule.
Claims20
A genetic sequence which codes for a single chain polypeptide having binding affinity for an antigen, said polypeptide comprising:
- (a) a first polypeptide comprising the binding portion of the light chain variable region of an antibody;
- (b) a second polypeptide comprising the binding portion of the heavy chain variable region of an antibody;
- and
- (c) at least one peptide linker linking said first and second polypeptides (a) and (b) into a single chain polypeptide having binding affinity for said antigen.
A method of producing a single chain polypeptide having binding affinity for an antigen, said polypeptide comprising:
- (a) a first polypeptide comprising the binding portion of the light chain variable region of an antibody;
- (b) a second polypeptide comprising the binding portion of the heavy chain variable region of an antibody;
- and (c) a peptide linker linking said first and second polypeptides (a) and (b) into said single chain polypeptide, said method comprising:
- (i) providing a genetic sequence coding for said single chain polypeptide;
- (ii) transforming a host cell with said sequence;
- (iii) expressing said sequence in said host;
- (iv) recovering said single chain polypeptide having binding affinity for an antigen.
Dependencies are inferred from the claim text.