Fruit specific transcriptional factors
AssigneeCalgene, Inc.
InventorsCatherine M. Houck, Julie R. Pear
Life of this patent
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Abstract
Fruit specific regulatory regions are identified employing cDNA screening. The resulting fruit specific regulatory regions are manipulated for use with foreign sequences for introduction into plant cells to provide transformed plants having fruit with a modified phenotypic property. The invention is exemplified with a tomato fruit specific promoter which is active throughout the stages of fruit ripening.
Claims14
A DNA construct comprising in the direction of transcription, a tomato 2All transcriptional initiation region joined to a DNA sequence of interest, wherein said DNA sequence of interest is other than the wild-type sequence and is under the transcriptional regulation of said 2All initiation region and a transcriptional termination region.
A DNA construct comprising in the direction of transcription, a tomato 2All transcriptional initiation region joined to a DNA sequence of interest, wherein said DNA sequence is other than the wild-type sequence and comprises a unique restriction site for insertion of a second DNA sequence of interest to be under the transcriptional regulation of said 2All initiation region, and a transcriptional termination region.
A method for modifying the phenotype of tomato fruit said method comprising:
- transforming a tomato plant cell with a DNA construct under genomic integration conditions, wherein said DNA construct comprises in the direction of transcription, a tomato 2All transcriptional initiation region jointed to a DNA sequence other than the wild-type sequence and capable of modifying the phenotype of fruit cells upon transcription, wherein said DNA sequence is under the transcriptional regulation of said initiation region and a transcriptional termination region, whereby said DNA construct becomes integrated into the genome of said tomato plant cell;
- regenerating a plant from said transformed tomato plant cell;
- and
- growing said plant to produce tomato fruit of the modified phenotype.
Tomato fruit comprising a DNA construct comprising in the direction of transcription, a tomato 2All transcriptional initiation region joined to a DNA sequence of interest, wherein said DNA sequence is other than the wild-type sequence and is under the transcriptional regulation of said 2All initiation region, and a transcriptional termination region.
A tomato plant comprising:
- a DNA construct comprising in the direction of transcription, a tomato 2All transcriptional initiation region joined to a DNA sequence of interest, wherein said DNA sequence is other than the wild-type sequence and is under the transcriptional regulation of said 2All initiation region, and a transciptional termination region.
A tomato plant comprising:
- a DNA construct comprising in the direction of transcription, a tomato 2All transcriptional initiation region joined to a DNA sequence of interest, wherein said DNA sequence is other than the wild-type sequence and comprises a unique restriction site for insertion of a second DNA sequence of interest to be under the transcriptional regulation of said 2All initiation region, and a transciptional termination region.
Dependencies are inferred from the claim text.