Reagent composition for fecal occult blood tests
AssigneeLitmus Concepts, Inc.
InventorPaul J. Lawrence
Life of this patent
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Abstract
Fecal occult blood tests of enhanced sensitivity, specificity, and readability are provided by employing (1) a developer or complete reagent solution that uses as a solvent system a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins such as dimethyl sulfoxide and/or (2) a multi-chromagen comprised of a mixture of guaiac and ABTS. Test results may be further improved by incorporating hemoprotein solubilizing agents, plant peroxidase inhibitors, iron/copper chelating agents, accelerators and buffers in the developer/reagent.
Claims34
A complete reagent composition for use in a fecal occult blood test comprising a solution of a leuco dye and a hydroperoxide in a solvent containing at least about 50% by volume of a solvent for iron protoporphyrins, wherein said solvent for iron protoporphyrins is a mixture of a neutralized amine and an organic co-solvent, wherein the neutralized amine is selected from the group consisting of ethanolamine, 2-(diethylamino)ethylamine, and diethanolamine.
A complete reagent composition for use in a fecal occult blood test comprising a solution of a leuco dye indicator and a hydroperoxide in a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins, wherein said solvent for iron protoporphyrins is pyridine, and wherein the pH of the solution is in the range of about 6.5 to about 7.5.
A complete reagent composition for use in a fecal occult blood test comprising a solution of a leuco dye indicator and a hydroperoxide in a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins, wherein said solvent for iron protoporphyrins is ethanolamine and a co-solvent selected from the group consisting of glycerol, methyl ethyl ketone, tetramethylene sulfone, butyrolactone, tetrahydrofurfuryl alcohol, 2-methoxy ethanol and tetramethyl urea, and wherein the pH of the solution is in the range of about 6.5 to about 7.5.
A complete reagent composition for use in a fecal occult blood test comprising a solution of a leuco dye indicator and a hydroperoxide in a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins, wherein said solvent for iron protoporphyrins is 2-(diethylamino)ethylamine and a co-solvent selected from the group consisting of methyl ethyl ketone, acetonitrile, tetramethylene sulfone, tetramethyl urea, butyrolactone, tetrahydrofurfuryl alcohol, 2-methoxy ethanol, and methanol, and wherein the pH of the solution is in the range of about 6.5 to about 7.5.
A complete reagent composition for use in a fecal occult blood test comprising a solution of a leuco dye indicator and a hydroperoxide in a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins, wherein said solvent for iron protoporphyrins is diethanolamine and a co-solvent selected from the group consisting of methyl ethyl ketone, acetonitrile, tetramethylene sulfone, butyrolactone, and tetrahydrofurfuryl alcohol, and wherein the pH of the solution is in the range of about 6.5 to about 7.5.
A complete reagent composition for use in a fecal occult blood test comprising a solution of a leuco dye indicator and a hydroperoxide in a solvent comprising at least about 50% by volume of a solvent for iron protoporphyrins, wherein said solvent for iron protoporphyrins is 1-methyl-2-pyrrolidinone, and wherein the pH of the solution is in the range of about 6.5 to about 7.5.
Dependencies are inferred from the claim text.