Polyredox couples in analyte determinations
AssigneeMolecular Devices, LLC
InventorsHenry G. Wada, Harden McConnell, Dean G. Hafeman
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Abstract
Enzyme or substrate determinations can be achieved by employing an organic second substrate, which produces a product which may be coupled with a metal inorganic redox couple capable of interacting with and affecting the potential of a metal electrode on a semiconductor surface. Particularly, hydrogen peroxide or indoxyl phosphate are coupled with iron- or ruthenium-containing ionic redox couples for determination with a noble metal electrode.
Claims7
In a method for determining the presence of an oxidase by means of employing a metal electrode for detection of a redox reaction in an assay medium, the improvement which comprises:
- employing for detection in combination:
- (1) a tetramethylbenzidine and hydrogen peroxide;
- with
- (2) an iron- or ruthemium-containing ionic redox couple in which said iron is in a coordinated form capable of undergoing one electron transfer and said ruthenium is coordinated with a nitrogen ligand
A method according to claim 11, wherein said oxidase is horseradish peroxidase and said hydrogen peroxide is produced by an enzymatic reaction during said assay.
A method according to claim 11 wherein said oxidase is horseradish peroxidase and said hydrogen peroxide is produced by the reaction of cholesterol oxidase or glucose oxidase.
A method according to claim 11, wherein said iron-containing ionic redox couple is the hexacyanoferrate redox couple.
A method according to claim 11, wherein said ruthenium-containing ionic redox couple is present as ruthenium pentaamine pyridine.
A method for determining cholesterol in a sample employing a metal electrode for detection of a redox reaction in an assay medium, said method comprising:
- combining said sample, cholesterol oxidase, horseradish peroxidase and tetramethylbenzidine with an iron- or ruthenium-containing ionic redox couple in which said iron is in a coordinated form capable of undergoing one electron transfer and said ruthenium is coordinated with a nitrogen ligand in an assay medium;
- and
- determining by means of a metal electrode the redox reaction in said assay medium and relating said reaction to a medium having a known amount of cholesterol.
A method for determining glucose in a sample employing a metal electrode for detection of a redox reaction in an assay medium, said method comprising:
- combining said sample, glucose oxidase, horseradish peroxidase and tetramethylbenzidine with an iron- or ruthenium-containing ionic redox couple in which said iron is in a coordinated form capable of undergoing one electron transfer and said ruthenium is coordinated with a nitrogen ligand in an assay medium;
- and
- determining by means of a metal electrode the redox reaction in said assay medium and relating said reaction to a medium having a known amount of glucose.
Dependencies are inferred from the claim text.