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US 4,942,032 AGranted
Process for producing a novel antifungal product
AssigneeMicrolife Technics, Inc.
InventorsPeter A. Vandenbergh, Blair S. Kunka
Life of this patent
Estimating the term…
Abstract
A method for producing a novel antifungal product from a Pediococcus species is described. The preferred product (AFP) comprises a compound which contains valine and lactic acid and has a molecular weight of less than about 500 daltons. The product (AFP) is particularly useful in retarding fungal growth in foods and other materials in need thereof.
Claims21
A method for producing an antifungal product (AFP) which comprises:
- (a) incubating live cells of a Pediococcus species in a growth medium for the cells so as to produce an isolable amount of the product (AFP) in the growth medium, wherein the product (AFP) produced in the growth medium or separated from the growth medium inhibits the growth of Penicillium oxalicum as an assay strain and wherein the product (AFP) comprises a compound as an essential ingredient consisting of valine and lactic acid having a molecular weight of about 400 daltons and less than about 500 daltons and has a FAB mass spectrum as shown in FIG. 2;
- and
- (b) treating the growth medium to eliminate most of the live cells of the Pediococcus species and to produce the product (AFP).
A method for producing an antifungal product (AFP) which comprises:
- (a) growing live cells of Pediococcus sp. in the presence of amino acid or peptide, protein, protein hydrolyzate or protein enzymatic digest in a growth medium;
- and
- (b) isolating the antifungal product (AFP) from the growth medium and eliminating most of the live cells of the Pediococcus sp, wherein the product (AFP) comprises a compound as an essential ingredient consisting of valine and lactic acid having a molecular weight of above 400 daltons and less than about 500 daltons, is digested by protease and inhibits the growth of Penicillium oxalicum as an assay strain in an optimal pH range between about pH 1 to 5 and has a FAB mass spectrum as shown in FIG. 2.
A method for producing an antifungal product (AFP) which comprises:
- (a) incubating live cells of a Pediococcus species in a growth medium for the cells containing growth factors present in cornsteep liquor, cysteine, garlic extract, milk digest, yeast extract, milk, egg or soy enzymatic digest or milk egg or soy hydrolyzate which induces the formation of the product (AFP), containing a protein source and containing a carbon source, so as to produce the product (AFP) in the growth medium;
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- (b) treating the growth medium which has been incubated to eliminate most of the live cells of the Pediococcus species so as to produce the product (AFP), wherein the product (AFP) inhibits the growth of Penicillium oxalicum spores as an assay strain in an assay with the product (AFP) and the Penicillium oxalicum spores mixed and wherein product (AFP) comprises a compound as an essential ingredient consisting of valine and lactic acid having a molecular weight of above 400 daltons and less than about 500 daltons and has a FAB mass spectrum as shown in FIG. 2.
A method for producing an antifungal product (AFP) which comprises:
- (a) incubating live cells of a Pediococcus species having antifungal characteristics of Pediococcus acidilactici ATCC 25742 in a growth medium for the cells containing growth factors present in cornsteep liquor, cysteine, garlic extract, milk digest, yeast extract, milk, egg or soy enzymatic digest or milk, egg or soy hydrolyzate or molasses which induce the formation of the product (AFP), containing a protein source and containing a carbon source so as to thereby produce product (AFP) in the nutrient medium;
- and
- (b) treating the growth medium which has been incubated to eliminate most of the live cells of the Pediococcus sp so as to produce the product (AFP), wherein the product (AFP) inhibits the growth of Penicillium oxalicum spores in an assay with the products (AFP) and the Penicillium oxalicum spores mixed and wherein product (AFP) comprises a compound as an essential ingredient consisting of valine and lactic acid having a molecular weight of above 400 daltons less than about 500 daltons and has a FAB mass spectrum as shown in FIG. 2.
A method for producing an antifungal product (AFP) which comprises:
- (a) incubating live cells of a Pediococcus species having antifungal characteristics of Pediococcus acidilactici ATCC 25742 in inhibiting the growth of Penicillium oxalicum as an assay strain in a growth medium for the cells containing growth factors present in cornsteep liquor, cysteine, garlic extract, milk digest, yeast extract, milk, egg or soy enzymatic digest or a milk, egg or soy hydrolyzate which induce the formation of the product (AFP) and containing a carbon source so as to produce the product (AFP) in the nutrient medium, wherein the product (AFP) inhibits the growth of Penicillium oxalicum spores as an assay strain in an assay with the product (AFP) and the Penicillium oxalicum spores mixed and wherein the product (AFP) comprises a compound as an essential ingredient consisting of valine and lactic acid having a molecular weight of about 400 daltons and less than about 500 daltons and has the FAB mass spectrum as shown in FIG. 2;
- (b) passing the incubated growth medium past a microporous filter means which allows the product (AFP) to pass through and which retains other components of the growth medium including the live cells of the Pediococcus species.
Dependencies are inferred from the claim text.