In vitro screening for and selection of glycine max resistant to phialophora gregata
AssigneesLubrizol Genetics, Inc., The Board of Trustees of the University of Illinois
InventorsYong-Quan Guan, Lynn E. Gray, Jack M. Widholm
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Abstract
An in vitro method for screening Glycine max cells for resistance to a disease caused by Phialophora gregata, brown stem rot, is disclosed. The method is useful for identifying brown stem rot resistant soybean varieties. Means for selecting mutant soybean cells for resistance to a toxin produced by P. gregata is also provided, as are cells and plants selected by this process. The invention involves culture of soybean calli in the presence of a filtrate of used fungal growth medium.
Claims19
A method for identifying the presence of brown stem rot resistance in a Glycine plant comprising growing cells of said plant in in vitro cell culture in the presence of a Phialophora gregata Type I-produced, species-specific, genotype-specific toxin, observing the viability of said cells, and identifying the presence of brown stem rot resistance in plants whose cultures show viability in the presence of said toxin.
A method for selection of a brown stem rot resistant variant soybean cell from an in vitro cell culture derived from a brown stem rot susceptible soybean plant comprising the steps of (a) culturing Glycine cells derived from the brown stem rot susceptible soybean plant such that the cell population comprises genetic variants, followed by (b) culturing the Glycine cells in the presence of a Phialophora gregata toxin, followed by (c) identifying and selecting a variant Glycine cell which is viable in the presence of said toxin as resistant to brown stem rot.
A method for producing a brown stem rot resistant Glycine plant which comprises the steps of
- (a) selecting a brown stem rot resistant variant soybean cell from an in vitro cell culture derived from a brown stem rot susceptible soybean plant by culturing Glycine cells derived from said susceptible soybean plant such that the cell population comprises variants, followed by culturing said Glycine cells in the presence of a Phialophora gregata toxin and identifying and selecting a variant Glycine cell which is viable in the presence of said toxin as resistant to brown stem rot, followed by
- (b) regenerating said brown stem rot resistant Glycine plant from said variant Glycine cell resistant to brown stem rot.
Dependencies are inferred from the claim text.