Method and device for ketone measurement
AssigneeAbbott Laboratories
InventorSamar K. Kundu
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Abstract
The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a matrix comprising a hydrazine compound coupled to an H.sup.+ ion exchange resin to produce a hydrazone reaction product with a characteristic color. Methods are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject by means of contacting said breath with a device comprising an H.sup.+ ion exchange resin to which a hydrazine compound has been coupled and correlating the breath acetone concentration to a standard reflecting the effect on breath acetone of fixed rates of fat metabolism.
Claims16
A method for the detection of ketone and aldehyde analytes in a fluid sample comprising:
- (a) reacting said analytes with a hydrazine compound coupled to an H.sup.+ ion exchange resin to form a colored reaction product,
- (b) fluting said reaction product from the ion exchange resin with a solvent, and
- (c) detecting the presence of a color signal in the eluted material.
A device for the detection of ketone and alkehyde analytes in a fluid sample comprising an H.sup.+ ion exchange resin to which a hydrazine compound has been coupled, and an adsorbent material.
A method for ascertaining the fat catabolism effects of a weight loss dietary regimen, said method comprising:
- (a) determining the breath acetone concentration of the subject by (i) contacting said breath with a device comprising an H.sup.+ ion exchange resin to which a hydrazine compound has been coupled to form a colored reaction product, (ii) eluting said reaction product from the ion exchange resin with a solvent, and (iii) detecting the presence of a color, and
- (b) correlating the breath acetone concentration to a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
A kit for the detection of ketone and aldehyde analytes in a fluid sample comprising:
- (a) an H.sup.+ ion exchange resin to which a hydrazine compound has been coupled;
- (b) a solid adsorbent;
- (c) solvent means for eluting a reaction product from the H.sup.+ ion exchange resin;
- and
- (d) means for detecting the presence of a color signal.
A kit for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising:
- (a) means for collecting a fixed volume of breath and contacting it with the solid adsorbent;
- (b) an H.sup.+ ion exchange resin to which 2,4-dinitrophenyl hydrazine has been coupled;
- (c) a solid adsorbent;
- (d) means for eluting said reaction
- (e) means for detecting the presence of a color signal;
- and
- (f) a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
Dependencies are inferred from the claim text.