Methods of using chemiluminescent 1,2-dioxetanes
AssigneeTropix, Inc.
InventorsIrena Y. Bronstein, John C. Voyta
Life of this patent
Estimating the term…
Abstract
Processes are disclosed in which light of different wavelengths is simultaneously released from two or more enzymatically decomposable chemiluminescent 1,2-dioxetane compounds, said compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of them, to each emit light of said different wavelengths, by decomposing each of said compounds by means of a different enzyme. Such processes can be used in multi-channel assays--immunoassays, chemical assays and nucleic acid probe assays--to detect the presence or determine the concentration of chemical or biological substances, and in multi-channel chemical/physical probe procedures for studying the microstructures of macromolecules.
Claims19
A process in which light of different wavelengths is simultaneously released from two or more enzymatically, chemically or electrochemically decomposable chemiluminescent 1,2-dioxetane compounds, at least one of said 1,2-dioxetane compounds being an enzymatically decomposable 1,2-dioxetane compound, said 1,2-dioxetane compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of said 1,2-dioxetane compounds, to each emit upon decomposition light of said different wavelengths, which comprises decomposing each of said 1,2-dioxetane compounds by means of one of two or more different enzyme, chemical or electrochemical decomposing means, each of said decomposing means being specific to one of said 1,2-dioxetane compounds, at least one of said decomposing means being an enzyme decomposing means.
Dependencies are inferred from the claim text.