Cell culture medium with antioxidant
AssigneeCell Enterprises, Inc.
InventorFrederick J. Darfler
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Abstract
Cells grown in chemically defined (serum free) media are protected against endogenous oxidizing agents by a nontoxic antioxidant with free thiol groups, at cell densities less than about 10.sup.5 cells/ml, especially DL-penicillamine, N-acetylcysteine, mercaptoproprionic acid, 2-mercaptoethanesulfonic acid, and thiolactate. These agensts are useful in the maintenance and growth of hybridomas in such media.
Claims26
A chemically defined, serum free, albumin-free medium for maintenance or growth of immortal or immortalized cells which comprises a cell-growth promoting amount of a compound having one free thiol group, and which is capable of supporting the growth of immortal or immortalized cells at seed densities less than 10.sup.5 cells/ml.
A chemically-defined, serum-free, medium for maintenance or growth of immortal or immortalized cells which comprises a cell growth promoting amount of a compound having one free thiol group, and which is capable of supporting the growth of immortal or immortalized cells at seed densities less than 10.sup.5 cells/ml.
A chemically defined, serum-free medium for the maintenance or growth of immortal or immortalized cells which comprises (a) a cell growth-promoting amount of a compound having one free thiol group, (b) insulin, and (c) transferrin, said medium being otherwise free of proteins.
A method of growing immortal or immortalized cells at seed densities less than 10.sup.5 cells/ml in a chemically defined serum-free medium which comprises inoculating a chemically defined, serum-free medium comprising a cell growth-promoting amount of a compound having one free thiol group with less than 10.sup.5 immortal or immortalized cells/ml medium and growing the cells in that medium.
A supplemental cell culture medium consisting essentially of transferrin, insulin, ethanolamine, a selenium salt, an unsaturated fatty acid, and a cell-growth-promoting amount of a compound having one free thiol group, where said medium, when added to a basal cell culture medium, supports the growth of hybridoma cells at seed densities of less than 10.sup.5 cells/ml.
Dependencies are inferred from the claim text.