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US 4,927,265 AGranted
Detector for fluorescence and absorption spectroscopy
Assignee501 Microphoretic Systems, Inc.
InventorRobert G. Brownlee
Life of this patent
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Abstract
Automated capillary electrophoresis tests can be performed using the capillary electrophoresis instrument of this invention. The capillary electrophoresis instrument has means for automatically purging and replenishing electrolytes, for automatically entering a sample into the capillary tube and for automatically performing capillary electrophoresis. A detector for simultaneous detection of absorbance and fluorescence provides means for quantifing the species separated by electrophoresis.
Claims21
A fluorescence and absorption detector comprising:
- light source means for simultaneously providing a first light beam and a second light beam wherein said first and second light beams are simultaneously incident upon and interact with a sample and emerging light from such a sample is characteristic of said sample;
- means, receiving said emerging light, for separating light into a number of different wavelengths wherein said emerging light is separated into different wavelengths;
- and
- means, receiving each of said different wavelengths from said means for separating light, for providing an output signal corresponding to each of said different wavelengths;
- wherein said first light beam includes a wavelength of light causing such a sample to fluoresce and said second light beam includes a wavelength absorbed by such a sample;
- said emerging light comprises unabsorbed light from said second light beam and fluorescent radiation;
- and
- a first subset of said output signals represent said flourescent radiation from such a sample and a second subset of said output signals represent said unabsorbed light from such a sample.
5 more elements
Dependencies are inferred from the claim text.