Process for producing proteins correlated with the diphtheric toxin
AssigneeSclavo S.p.A.
InventorRino Rappuoli
Life of this patent
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Abstract
Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml, at a temperature of from 30.degree. C. to 40.degree. C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.
Claims3
A process for the production of protein cross reacting with diptheria toxin comprising the step of culturing in a liquid nutrient medium with a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml and at a temperature of from 30.degree. C. to 40.degree. C. at a neutral pH and under aerobic conditions, a microorganism belonging to Corynebacterium diphtheriae with two mutant phages encoding for the protein cross reacting with diphtheria toxin and integrated nontandemly at attachment sites B.sub.1 and B.sub.2 of the bacterial chromosome wherein the protein is CRM 197 protein and the microorganism is the C.sub.7 (197) M, ATCC-39255 strain with two 197 mutant phages integrated nontandemly in said attachments sites B.sub.1 and B.sub.2.
A process for the production of protein cross reacting with diptheria toxin comprising the step of culturing in a liquid nutrient medium with a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml and at a temperature of from 30.degree. C. to 40.degree. at a neutral pH and under aerobic conditions, a microorganism belonging to Corynebacterium diphtheriae with two mutant phages encoding for the protein cross reacting diphteria toxin and integrated nontandemly at attachment sites B.sub.1 and B.sub.2 of the bacterial chromosome wherein the protein is CRM45 protein and the microorganism is the C.sub.7 (45) M.sub.8 ATCC 39526 strain with two mutant 45 phages integrated nontandemly in said attachment sites B.sub.1 and B.sub.2.
Dependencies are inferred from the claim text.