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US 4,923,978 AGranted
Process for purifying nucleic acids
AssigneeE. I. Du Pont de Nemours and Company
InventorRandy M. McCormick
Life of this patent
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Abstract
The process for separating the proteinaceous materials from nucleic acids involves contacting a solution containing the proteinaceous materials and nucleic acids with a solid phase extraction material capable of binding proteins to form bound and unbound fractions and then isolating the unbound fraction containing the nucleic acids.
Claims4
A process for separating enzymes from nucleic acids which comprises
- a. contacting a solution containing enzymes and nucleic acids with a rehydrated silica gel said rehydrated silica gel having a specific surface area of 50 to 150 m.sup.2/ g and pores throughout having an effective diameter of 60 .ANG. to 150 and having surface hydroxyl groups at a concentration of 0.1 .mu.mol/m.sup.2 to 8 mol/m.sup.2 and at a pK.sub.a between 6 and 10 capable of binding enzymes to leave an unbound fraction containing the nucleic acids;
- and
- b. isolating the unbound fraction.
Dependencies are inferred from the claim text.