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US 4,921,801 AGranted
Novel recombinant DNA cosmid shuttle vectors
AssigneeEli Lilly and Company
InventorsR. Nagaraja Rao, Richard K. Stanzak
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Abstract
Novel recombinant DNA cosmid shuttle vectors and a method of using them in the construction of genomic DNA libraries are described. The vectors demonstrate the incorporation of both the size selection and in vitro packaging mechanisms of lambda into a Streptomyces-E. coli shuttle vector by the incorporation of two or more COS sequences of bacteriophage lambda.
Claims58
A recombinant double standard DNA cosmid shuttle vector comprising:
- (a) a replicon that is functional in E. coli,
- (b) a replicon that is functional in Streptomyces,
- (c) a double stranded DNA segment that contains two or more cos sequences of bacteriophage lambda, and
- (d) one or more DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive restrictionless host cell.
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