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US 4,918,009 AGranted
Method of controlling the regioselectivity of glycosidic bonds
AssigneeSvenska Sockerfabriks AB
InventorKurt G. I. Nilsson
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AssigneeSvenska Sockerfabriks AB
InventorKurt G. I. Nilsson
Estimating the term…
A method of controlling the regioselectivity of the glycosidic bond between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the cabohydrate part in a glycoconjugate, by reverse hydrolysis or transglycosidation reactions, is described. The synthesis is carried out in that a donor substance which is a mono- or oligosaccharide or a glycoside thereof, is caused to react, in the presence of a glycosidase, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, the .alpha. or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and the oligosaccharide compound being separated from the reaction mixture.
A method of controlling the regioselectivity of the glycosidic bond formed between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the carbohydrate part in a glycoconjugate, synthesized by reverse hydrolysis or transglycosidation, comprising reacting a donor substance which is a monosaccharide or oligosaccharide or a glycoside of a monosaccharide or oligosaccharide, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, in the presence of an exo- or endoglycosidase of EC group 3.2, the .alpha.- or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and separating the oligosaccharide compound from the reaction mixture.
Dependencies are inferred from the claim text.