Eucaryotic expression system
AssigneeGenetics Institute, Inc.
InventorsRandal J. Kaufman, Andrew Dorner
Life of this patent
Estimating the term…
Abstract
This invention provides vectors, improved host cells and improved methods for producing a heterologous protein by culturing an improved eucaryotic host cell of this invention transformed or transfected with a vector capable of directing the expression of the heterologous protein. The preferred improved host cell of this invention is a mammalian host cell containing and capable of expressing an anti-sense GRP78 DNA sequence.
Claims7
An anti-sense expression vector capable of directing the transcription in a host cell of mRNA complementary to mRNA encoding the host cell's GRP78, said vector comprising a DNA sequence encoding host cell GRP78 peptide sequence operatively linked to an expression control sequence in reverse orientation such that transcription of the DNA produces the anti-sense mRNA.
Dependencies are inferred from the claim text.