US 4,910,148 A · Magnetic separation of magnetized particles from biological fluids

Abstract

A method and device are provided for separating magnetized particles from biological fluids. Cells such as cancer cells coated with magnetized particles can be separated from uncoated healthy cells. A fluid mixture of cancer cells, healthy cells and magnetizable particles is introduced into a container such as a disposable blood bag which is attached in a cassette on an underlying plane magnetic plane that provides a magnetic field. Incubation is carried out during which the cancer cells become coated with the magnetizable particles. The magnetic field pulls the coated cells down towards the bottom of the bag and anchors them, and uncoated healthy cells are removed from the bag. The separated uncoated healthy cells may be advanced through a final separation unit where any loose magnetizable particles are removed. There is provided means for adjusting vertical distance between the cassette and the magnetic plane and for agitating fluid within the container attached to the cassette. A magnetic screen plate may be placed between the cassette and the magnetic plate to short circuit the magnetic field during incubation. The screen plate is removed to carry out separation.

Claims14

Dependent claims

A device for separating magnetized particles from biological fluids in which said magnetized particles are suspended, said device comprising:

(a) a sample container having an inlet means for introducing biological fluids thereinto and an outlet means for withdrawing biological fluids therefrom,
(b) a magnetic plate means comprising a plurality of low profile, high field density, permanent magnets, wherein the plurality of magnets are oriented on a generally horizontal base plate such that each magnet is spaced from the others and is adjacent magnets having an opposite polarity,
(c) a sample cassette means for holding said sample container in a generally vertical position, said sample cassette means being located above, and generally parallel to, said magnetic plate means,
(d) an adjusting means, operatively connected to said sample cassette means and said magnetic plate means, for adjusting the vertical distance between said sample cassette means and said magnetic plate means, and
(e) a rocker support means for agitating the fluid contents within said sample container.

2 more elements

A method for separating at least a portion of magnetized particles from biological fluids in which magnetized particles are suspended, said method being carried out with a device which comprises:

a sample container having an inlet means for introducing biological fluids therein and an outlet means for withdrawing biological fluids therefrom;
a magnetic plate means comprising a plurality of low profile, high field density, permanent magnets, wherein the plurality of magnets are oriented on a generally horizontal base plate such that each magnet is spaced from the others and is adjacent magnets having an opposite polarity;
a sample cassette means for holding said sample container in a generally vertical position, said sample cassette means being located above and generally parallel to said magnetic plate means;
an adjusting means, operatively attached to said sample cassette means and said magnetic plate means, for adjusting the vertical distance between said cassette means and said magnetic plate means;
and a rocker support means for agitating the fluid contents within said sample container, said method comprising the steps of:
(a) partially filling said sample container through said inlet means with a biological fluid in which are suspended magnetized particles,
(b) attaching said sample container to said sample cassette means,
(c) adjusting the distance between said sample cassette means and said magnetic plate means with said adjusting means while simultaneously agitating the fluid contents within said sample container with said rocker support means, and
(d) withdrawing said biological fluid through said sample container outlet means while retaining said magnetized particles by said magnetic plate means with said container.

6 more elements

Dependencies are inferred from the claim text.

Magnetic separation of magnetized particles from biological fluids

Life of this patent

Magnetic separation of magnetized particles from biological fluids

Life of this patent

Abstract

Claims14