Novel polypeptide having gamma-interferon activity
AssigneeNew York University
InventorYum K. Yip
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Abstract
The present invention provides a peptide having an amino acid sequence of less than about 100 amino acids, immunochemically reactive with an antibody directed against human gamma-interferon, and displaying gamma-interferon antiviral and cytolytic activities.
Claims11
A process for purifying a peptide having an amino acid sequence less than 100 amino acids, said peptide being immunochemically reactive with an antibody to gamma-interferon and having antiviral and cytolytic activities substantially the same as those of gamma-interferon, from an impure preparation comprising said peptide and also comprising gamma-interferon and other proteins as impurities;
- said process comprising the steps of:
- subjecting said preparation to adsorption onto silicic acid thereby causing said gamma-interferon and said peptide to bind to said silicic acid;
- desorbing said peptide and said gamma-interferon from said silicic acid;
- subjecting said desorbate to anion exchange chromatography, thereby causing impurities to bind to said column while said peptide and said gamma-interferon are excluded in a portion of the chromatographic effluent;
- subjecting said portion of said effluent to high-separation-speed molecular sieve chromatography under non-denaturing conditions thereby causing resolution of said gamma-interferon and said peptide;
- recovering fractions containing said peptide;
- subjecting said fractions containing said peptide to chromatography on a cation exchange residue, thereby causing said peptide to bind to said cation exchanger, and
- eluting and recovering said peptide from said cation exchanger.
A process for purifying gamma-interferon from an impure preparation containing said gamma-interferon and other proteins as impurities, comprising the steps of:
- subjecting said preparation containing said gamma-interferon to adsorption onto silicic acid;
- desorbing said gamma-interferon;
- subjecting said desorbate to anion exchange chromatography, thereby excluding said gamma-interferon in a portion of the chromatographic effluent;
- subjecting said portion of said effluent to high-separation-speed molecular sieve chromatography under non-denaturing conditions thereby causing resolution of said gamma-interferon;
- recovering fractions containing said gamma-interferon;
- subjecting said fractions containing said gamma-interferon to chromatography on a cation exchanger, thereby causing said gamma-interferon to bind to said cation exchanger, and
- eluting and recovering said gamma-interferon from said anion exchanger.
A process for purifying a peptide having an amino acid sequence less than 100 amino acids from an impure preparation containing said peptide, gamma-interferon, and other proteins as impurities, said peptide being immunochemically reactive with an antibody to gamma-interferon and having antiviral and cytoltic activities substantially the same as those of gamma-interferon, said process comprising the steps of:
- (1) adsorbing said peptide and gamma-interferon on silicic acid from said impure preparation;
- (2) removing said impurities from said adsorbed peptide and gamma-interferon by anion-exchange chromatography to form an impurity-free solution;
- (3) exposing said impurity-free solution to high-separation-speed molecular sieve chromatography under non-denaturing conditions to form a peptide-containing solution free of gamma interferon;
- and
- (4) recovering said peptide by cation-exchange chromatography.
Dependencies are inferred from the claim text.