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US 4,908,321 AGranted
Radio-active method for determining trace amounts of peroxide
AssigneeNone recorded
InventorShambhu D. Varma
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Abstract
There is disclosed a method of contacting a peroxide containing substance with non-volatile, alpha-keto acid radiolabeled with --C.sup.14 in the carboxyl group and determining radioactivity evolved .sup.14 CO.sub.2 and thereby calculating the amount of peroxide in the substance.
Drawings
Claims3
A method for determining the presence of peroxides in less than micromolar amounts contained in a biological and/or non-biological sample, which comprises:
- (a) contacting a suspect sample with a non-volatile acid with the formula, ##STR2## wherein R is an alkyl hydrocarbon or its derivative with from 1 to 6 carbon atoms;
- (b) trapping liberated .sup.14 CO.sub.2 ;
- (c) determining liberated .sup.14 CO.sub.2 by scintillation counting;
- and
- (d) evaluating the results of step (c) to determine the peroxide content of said sample.
2 more elements
Dependencies are inferred from the claim text.