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US 4,908,318 AGranted
Nucleic acid extraction methods
AssigneeIntegrated Genetics, Inc.
InventorTerry Lerner
Life of this patent
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Abstract
A method of extracting nucleic acids from a crude buffy coat fraction in an aqueous medium includes the steps of treating the buffy coat fraction to break open the white blood cells to release the proteins and the nucleic acids in the cells into the aqueous medium; solubilizing the proteins and the nucleic acids in the aqueous medium; and precipitating the nucleic acids under conditions under which the proteins remain in solution.
Claims7
A method of extracting nucleic acids from a crude buffy coat fraction in an aqueous medium, comprising the steps of:
- (a) first treating said crude buffy coat fraction with biological detergent means to cause white blood cells contained in said fraction to release proteins and nucleic acids contained within said cells into said aqueous medium;
- (b) solubilizing said proteins and said nucleic acids in said aqueous medium from step (a) by the addition of a solubilizing agent;
- (c) precipitating said solubilized nucleic acids of step (b) with a nucleic precipitating agent under conditions whereby said proteins remain in solution;
- and
- (d) spooling said precipitated nucleic acids.
2 more elements
Dependencies are inferred from the claim text.