Process for producing phenylananine
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsAkio Ozaki, Ryoichi Katsumata, Tetsuo Oka
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Abstract
The invention relates to a bacterial process for producing phenylalanine. The process utilizes a Corynebacterium or Brevibacterium host which is transformed with a recombinant DNA. The recombinant DNA harbors a DNA containing a gene which encodes chorismate mutase or prephenate dehydratase. The transformed microorganism is then cultured in order to accumulate phenylalanine in the culture medium and the phenylalanine is recovered therefrom.
Claims5
A process for producing phenylalanine which comprises transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA comprising a DNA fragment containing a gene coding for at least one of chorismate mutase and prephenate dehydratase obtained from a microorganism belonging to the genus Corynebacterium or Brevibacterium, culturing the transformant in a nutrient medium containing a molasses carbon source, accumulating phenylalanine in the culture medium and recovering phenylalanine therefrom.
An isolated DNA fragment or a recombinant DNA containing the DNA fragment, which DNA fragment or recombinant DNA contains a gene coding for at least one of chorismate mutase and prephenate dehydratase obtained from a microorganism belonging to the genus Corynebacterium or Brevibacterium.
A microorganism belonging to the genus Corynebacterium or Brevibacterium and having a native ability to produce phenylalanine, said microorganism containing a recombinant DNA comprising a DNA fragment containing a gene coding for at least one of chorismate mutase and prephenate dehydratase, said gene obtained from a microorganism belonging to the genus Corynebacterium or Brevibacterium.
Corynebacterium glutamicum K39, FERM P-7088 (FERM BP-459).
Dependencies are inferred from the claim text.