Competitive elisa for determination of neutralizing IBDV antibody
AssigneeUniversity of Maryland
InventorDavid B. Snyder
Life of this patent
Estimating the term…
Abstract
A method for determining the level of infectious bursal disease virus IBDV neutralizing antibody in poultry comprising a labeled monoclonal antibody R63 having the IBDV neutralizing capability of the monoclonal antibody expressed by hybridoma cell line ATCC HB-9490. Monoclonal antibody R63 is specific to all known IBDV strains and serotypes and competes only with itself and other antibodies recognizing the same neutralizing epitope present in poultry sera. In a competition assay, the increase in unbound labeled monoclonal antibody is an indication of the level of IBDV neutralizing antibody present in poultry sera.
Claims9
A method for determining the level of infectious bursal disease virus (IBDV) neutralizing antibody in poultry, comprising:
- A, admixing (1) a predetermined amount of labeled monoclonal antibody having the IBDV neutralizing capability of the monoclonal antibody expressed by hybridoma cell line ATCC-HB-9490, (2) infectious bursal disease virus antibody-containing fluid from said poultry, and (3) a predetermined amount of infectious bursal disease virus antigen;
- B, determining the amount of labeled monoclonal antibody not bound to said antigen in said admixing step, by measurement of the label attached to either said unbound monoclonal antibody or the label attached to monoclonal antibody bound to said antigen,
- C, comparing said amount of unbound labeled monoclonal antibody with a standard value for the amount of labeled monoclonal antibody remaining unbound in the absence of said poultry fluid as a measure of the level of IBDV neutralizing antibody in said fluid.
A kit for conducting a competitive ELISA assay comprising, in separate containers, monoclonal antibody having the IBDV neutralizing capability of the monoclonal antibody expressed by hybridoma cell line ATCC HB-9490 bound to a label, and a predetermined amount of infectious bursal disease virus antigen.
Dependencies are inferred from the claim text.