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US 4,908,013 AGranted
Holder for a vein segment and method of removing cells from a vein segment
AssigneeSulzer Brothers Limited
InventorsWerner Muller, Marie-Claude Hensel, August Huber
Life of this patent
Estimating the term…
Abstract
The holder contains a sealed chamber in which a vein segment is clamped centrally with a slight axial prestress. For storage and transportation, the interior of the vein segment and the surrounding peripheral portions of the chamber housing the vein segment can be filled with a physiological common salt solution via flow channels. For removal of endothelial cells, an enzyme solution can be passed through the flow channels aligned with the vein segment to entrain the endothelium cells therein for subsequent processing.
Claims20
A holder for a vein segment comprising
- a base having a pair of end walls defining a laterally accessible recessed chamber for receiving a vein segment;
- a closure element mounted on said base in hermetically sealed relation to close said chamber;
- a pair of flow channels each flow channel being disposed in a respective wall of said base and communicating said chamber with the exterior of said base;
- a vein segment holding and tensioning means disposed in said chamber for mounting a vein segment between and in communication with said flow channels for passage of fluid therebetween;
- and
- at least a third flow channel in said base communicating a periphery of said chamber with the exterior of said base.
3 more elements
A holder for a vein segment comprising
- a base having a pair of end walls defining a laterally accessible recessed chamber for receiving a vein segment;
- removable closure element mounted on said base to close said chamber;
- a vein segment holding and tensioning means disposed in said chamber for mounting a vein segment in tensioned relation thereon;
- a pair of flow channels for communicating an interior of a vein segment mounted on said holder and tensioning means with the exterior of said base for passage of fluid therebetween each flow channel being disposed in a respective wall of said base;
- and
- a third flow channel communicating a periphery of said chamber with the exterior of said base.
3 more elements
In a method of removing cells from a vein segment, the steps comprising
- mounting the vein segment under longitudinal tension in a sealed chamber;
- communicating the opposite ends of the vein segment with a means for passing a flushing solution therethrough;
- flushing the solution through the vein segment to remove blood residue;
- filling the chamber about the vein segment with a physiological common salt solution to prevent drying of the vein segment;
- and
- communicating the periphery of the chamber with a buffer space having an air cushion therein to define a pressure equalization vessel.
3 more elements
A holder for a vein segment comprising
- a base of circular cylindrical shape having a chamber for receiving a vein segment opening laterally of said base, said base having a shoulder at one end;
- a closure element abutting said shoulder and mounted on said base in hermetically sealed relation to close said chamber;
- a pair of flow channels in said base communicating said chamber with the exterior of said base, each said flow channel terminating in a respective end face of said base;
- a vein segment holding and tensioning means disposed in said chamber for mounting a vein segment between and in communication with said flow channels for passage of fluid therebetween;
- and
- at least a third flow channel in said base communicating a periphery of said chamber with the exterior of said base.
3 more elements
A holder for a vein segment comprising
- a base of circular cylindrical shape having a chamber for receiving a vein segment opening laterally of said base;
- a sleeve mounted on said base in hermetically sealed relation to close said chamber;
- a pair of flow channels in said base communicating said chamber with the exterior of said base, each said flow channel terminating in a respective end face of said base;
- a vein segment holding and tensioning means disposed in said chamber for mounting a vein segment between and in communication with said flow channels for passage of fluid therebetween;
- and
- at least a third flow channel in said base communicating a periphery of said chamber with the exterior of said base.
3 more elements
A holder for a vein segment comprising
- a base having a chamber for receiving a vein segment;
- a closure element mounted on said base in hermetically sealed relation to close said chamber;
- a pair of flow channels in said base communicating said chamber with the exterior of said base;
- a vein segment holding and tensioning means disposed in said chamber for mounting a vein segment between and in communication with said flow channels for passage of fluid therebetween;
- a pair of spigots, each spigot terminating each respective flow channel and having a cone-shaped bore for matingly receiving an end of a syringe;
- a pair of caps, each said cap being removably mounted on a respective spigot;
- and
- at least a third flow channel in said base communicating a periphery of said chamber with the exterior of said base.
5 more elements
A holder for a vein segment comprising
- a base having a chamber for receiving a vein segment;
- a closure element mounted on said base in hermetically sealed relation to close said chamber;
- a pair of flow channels in said base communicating said chamber with the exterior of said base;
- a vein segment holding and tensioning means disposed in said chamber for mounting a vein segment between and in communication with said flow channels for passage of fluid therebetween, said means including a pair of hollow connectors for engaging with opposite ends of a vein segment and a pair of elongated tubes, each said tube receiving one of said connectors thereon in axial alignment and being slidably mounted in said base in communication with a respective one of said flow channels;
- and
- at least a third flow channel in said base communicating a periphery of said chamber with the exterior of said base.
3 more elements
Dependencies are inferred from the claim text.