Culture medium for detection of beer spoilage microorganisms
AssigneeMiller Brewing Company
InventorsMichael C. Barney, Edward J. Kot, Etzer Chicoye
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Abstract
Beer spoilage microorganisms are detected with a culture medium that is advantageous for detecting Lactobacillus spp., Pediococcus spp. and wild yeast, and which allows detection to be carried out in four days. The medium contains tomato juice, peptones, cysteine hydrochloride, sorbitan nonooleate, mono and disaccharides, yeast extract, beef extract, potassium acetate, malic acid, mono and dibasic potassium phosphate, magnesium sulfate, maganese sulfate, sodium chloride, ferrous sulfate, beer, water and agar.
Claims1
A culture medium for growing beer spoilage microorganisms that may be present in beer, said medium containing the following ingredients in about the concentrations given:
- ______________________________________ (w/v) ______________________________________ tomato juice 0.70% polypeptone 0.50% yeast extract 0.37% beef extract 0.20% maltose 1.50% dextrose 1.37% potassium acetate 0.30% sorbitan mono-oleate 0.05% monopotassium phosphate 0.02% dipotassium phosphate 0.02% cysteine hydrochloride 0.02% malic acid 0.05% magnesium sulfate 0.007% manganese sulfate 0.0004% sodium chloride 0.0004% ferrous sulfate 0.0004% agar 1.50% beer 25.00% water 68.38% ______________________________________
Dependencies are inferred from the claim text.