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US 4,906,571 AGranted
Cell surface modification using a novel glycoproteinase of pasteurella haemolytica
AssigneeUniversity of Guelph
InventorsAlan Mellors, Alphonsus E. Udoh
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Abstract
A glycoproteinase derived from the culture of Pasteurella haemolytica cleaves carbohydrate antigen markers from glycoproteins forming an integral part of plant and mammal cell membrane without general enzyme digestion of other cell protein. The glycoproteinase is separated from the media containing nutrients which induce Pasteurella haemolytica to produce the glycoproteinase released into the media.
Claims6
A glycoproteinase, isolated and purified from culture media of Pasterurella haemolytica having the following identifying characteristics whereby said glycoproteinase:
- (i) cleaves glycophorin A on the carboxylate side of histidine at amine acid residue 66 or 67 of said glycophorin as shown in FIG. 1 without general enzyme digestion of other cell protein,
- (ii) is inhibited by metal chelators, and
- (iii) has optimum enzymatic activity at a pH in the range of 7 to 8.5.
Dependencies are inferred from the claim text.