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US 4,906,567 AGranted
Non-immunochemical binding of lipopolysaccharides and sandwich assays therefor
AssigneeE. I. DuPont de Nemours and Company
InventorMark C. Connelly
Life of this patent
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Abstract
Sandwich assays for detecting and identifying lipopolysaccharides of gram negative bacteria utilizing immobilized lipopolysaccharide binding proteins and labelled detection reagents are provided. The active supports are also useful in removing LPS from biomedical and cosmetic preparations.
Claims8
A sandwich assay for detecting lipopolysaccharides comprising the steps of:
- (A) immobilizing a specific, non-immunochemical capture reagent in a substantially surfactant-free environment below pH=9 to form an active support consisting essentially of
- (i) a water insoluble support;
- and
- (ii) lipopolysaccharide binding proteins of amebocyte lysates bound to said support;
- (B) contacting a substantially surfactant-free sample containing or suspected of containing lipopolysaccaharides with said active support;
- (C) contacting the active support-LPS complex formed in step (B) with a substantially surfactant-free labelled detection reagent;
- and
- (D) detecting either the bound or unbound label.
5 more elements
A method for removing lipopolysaccharides from a sample comprising the steps of:
- (A) immobilizing a specific, non-immunochemical capture reagent in a substantially surfactant-free environment below pH=9 to form an active support consisting essentially of
- (i) a water insoluble support;
- and
- (ii) lipopolysaccharide binding proteins of amebocyte lysates bound to said support;
- (B) contacting a substantially surfactant-free sample containing or suspected of containing the lipopolysaccharide with the active support;
- and
- (C) separating the LPS bound to the active support from the sample.
4 more elements
Dependencies are inferred from the claim text.