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US 4,906,564 AGranted
Antigenic determinants recognized by antibodies obtained using a pathogenic agent or a derivative thereof that presents a restricted set of antigens
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsJeffery A. Lyon, Jeffrey L. Chulay, Alan W. Thomas, Russell J. Howard, James L. Weber
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Abstract
A method provides peptides that are antigenic determinants identified by antibodies obtained using intact pathogenic agents that present a restricted set of antigens to surveillance by the immune system.
Claims8
A method for selectively identifying surface-accessible antigens and surface accessible antigenic determinants of an intact pathogenic agent, comprising the steps of:
- (A) providing a sample comprising a plurality of intact units of a pathogenic agent,
- (B) contacting said sample with a heterogeneous mixture of antibodies from immune serum, such that antibodies from said mixture bind to antigenic determinant forming immune complexes on an intact unit of said plurality,
- (C) isolating said antibodies from said immune complexes,
- (D) obtaining defined fractions of a heterogeneous mixture of antigens produced by said pathogenic agent,
- (E) contacting said antibodies from step (C) with the separated heterogeneous mixture of antigens from step (D), and
- (F) identifying said separated antigens recognized by said antibodies.
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Dependencies are inferred from the claim text.