Detection of skatole for meat quality
AssigneeIdetek, Inc.
InventorsPrithipal Singh, Ashoke Sinha
Life of this patent
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Abstract
A method for detecting boar taint in a pig sample is provided. Anti-skatole antibodies are combined in a reaction buffer with a pig sample to form a reaction mixture. After incubating, the amount of skatole-antibody complex formation is determined. In a particular embodiment, the method utilizes solid substrate-affixed antibodies in a competitive inhibition assay with an enzyme-labeled skatole analogue. Kits facilitating the method are also provided.
Claims21
A method for detecting boar taint in a pig comprising:
- (a) combining in a reaction buffer a sample from said pig and anti-skatole antibodies to form a reaction mixture;
- (b) incubating said reaction mixture for a period of time sufficient to form an antibody-skatole complex with any skatole present in said reaction mixture;
- and
- (c) determining the amount of antibody-skatole complex as an indication of boar taint in said pig.
A method for detecting boar taint in a pig comprising:
- (a) combining in a reaction buffer a blood sample from said pig and anti-skatole antibodies affixed to a solid support to form a reaction mixture;
- (b) incubating said reaction mixture for a period of time sufficient to form an antibody-skatole complex with any skatole present in said reaction mixture;
- (c) washing said solid support;
- and
- (d) determining the amount of antibody-skatole complex affixed to said solid support as an indication of boar taint in said sample.
A method for detecting boar taint in a pig comprising:
- (a) combining in a reaction buffer a labeled skatole analogue, a blood sample from said pig and anti-skatole antibodies affixed to a solid support to form a reaction mixture;
- (b) incubating said reaction mixture for a period of time sufficient to form an antibody-skatole complex with any skatole present in said reaction mixture;
- (c) washing said solid support;
- and
- (d) determining the amount of said label bound to said solid support in comparison to a sample having a known amount of skatole as an indication of boar taint in said pig.
A method for detecting boar taint in a pig comprising:
- (a) combining in a reaction buffer a conjugate of an enzyme with skatole or a skatole analogue, a blood sample from said pig, and anti-skatole antibodies affixed to a solid support to form a first reaction mixture;
- (b) incubating said first reaction mixture for a time period sufficient to form an antibody-skatole complex;
- (c) washing said solid support with a wash buffer;
- (d) incubating a solution containing a substrate for said enzyme with said solid support to form a second reaction mixture;
- and
- (e) determining the optical density of said second reaction mixture in comparison to the optical density of sample having a known amount of skatole as an indication of boar taint in said pig.
A method for detecting boar taint in a pig comprising:
- (a) combining a blood sample from said pig, indole-3-butyric acid/alkaline phosphatase conjugate in Tris-buffered saline, and anti-skatole antibodies affixed to a microtiter plate to form a first reaction mixture;
- (b) incubating said reaction mixture at about 4.degree. C. for about four hours;
- (c) washing said microtiter plate with salinepolyoxylenesorbitan buffer;
- (d) incubating a p-nitrophenylphosphate solution with said microtiter plate at about 37.degree. C. for about one hour to form a second reaction mixture:
- (e) altering the pH of said second reaction mixture to stop enzyme activity to form a third reaction mixture;
- and
- (f) determining the optical density of said third reaction mixture in comparison to the optical density of a sample having a known amount of skatole to determine the skatole concentration of said sample,
- wherein said pig is identified as having boar said sample has greater than about 0.3 ppm skatole.
A kit for determining boar taint in a pig blood sample comprising in separate containers (a) anti-skatole antibodies and (b) labeled skatole or a labeled skatole analogue.
Dependencies are inferred from the claim text.