Antimicrobial compound isolated from Actinomadura fulva subsp indica

#This invention relates to a macrolactam disaccharide antimicrobial compound. The compound is isolated from an antimicrobial complex 530 which is produced in fermentation under controlled conditions using a biologically pure culture of the microorganism, Actinomadura fulva subsp. indica SCC 1840, ATCC 53714.

#In a related, commonly assigned, co-pending application Ser. No. 227,964, filed on Aug. 3, 1988, three novel macrolactam monosaccharides produced by fermentation of A. fulva subsp. uruguayensis ATCC 53713 are disclosed.

#In another related, commonly-assigned, co-pending application Ser. No. 227,968 filed on Aug. 3, 1988, a novel macrolactam monosaccharide produced by fermentation of A. vulgaris subsp. vulgaris sp nov, ATCC 53748 are disclosed.

#Prepare a 250 mL Erlemneyer flask with 70 mL of the following germination medium:

#______________________________________ Beef Extract 3 g Tryptone 5 g Yeast Extract 5 g Cerelose 1 g Potato Starch 24 g Calcium Carbonate 2 g Tap Water 1000 mL AF-1* 1 mL ______________________________________ *AF-1 is an antifoam agent available from Dow Corning Corp., Midland, MI 48641.

#Adjust the pH of the germination broth to 7.5. Sterilization the broth and after cooling, add 3.5 mL of a frozen whole broth sample of the microorganism of this invention from a previously prepared inoculum to each flask broth. Incubate at 30.degree. C. with continual agitation of 300 rpm for 48 hours.