DNA fragments containing alkaline cellulase gene, recombinant plasmids with said DNA fragments inserted therein, and recombinant microorganisms
AssigneeKao Corporation
InventorsKatsuya Ozaki, Kazushi Oshino, Kenzo Koike, Susumu Ito, Kikuhiko Okamoto
Life of this patent
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Abstract
DNA frangments containing an alkaline cellulase K gene, which comprise about 4.0, about 2.4 and about 1.9 kilo base pairs, respectively, each having a specific restruction map, recombinant plasmids containing any of these DNA fragments, and recombinant microorganisms harboring any of said plasmids. The alkaline cellulase K gene is derived from a bacterial strain which belongs to the genus Bacillus and is alkalophilic, that is, capable of revealing an optimum growth in an alkaline pH region.
Claims9
A DNA fragment coding for alkaline cellulase K which comprises about 4.0 kilo base pairs and has the restriction map shown in FIG. 1A. wherein said DNA fragment is isolated from Bacillus sp. Ksm-635.
A recombinant plasmid which contains a DNA fragment coding for alkaline cellulase K wherein said DNA fragment comprises about 4.0 kilo base paris and has the restriction map shown in FIG. 1, wherein said DNA fragment is isolated from Bacillus sp. Ksm-635.
A transformed microorganism carrying a recombinant plasmid which contains a DNA fragment coding for alkaline cellulase K wherein said DNA fragment comprises about 4.0 kilo base pair and has the restriction map shown in FIG. 1, wherein said DNA fragment is isolated from Bacillus sp. Ksm-635.
Dependencies are inferred from the claim text.