Site-specific homogeneous modification of polypeptides
AssigneeGenetics Institute, Inc.
InventorGray Shaw
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Abstract
A homogeneously modified protein is provided having one or more selected naturally occurring lysine residues replaced by a suitable amino acid, or having one or more lysine residues substituted for other amino acids or inserted into a polypeptide sequence, leaving selected lysine residues having .epsilon.-amino groups in the protein and coupling amine reactive compounds to selected lysine residues. Methods for producing the selected homogeneously modified proteins and pharmaceutical compositions containing such proteins are provided.
Claims3
A polypeptide selected from the group consisting of G-CSF and erythropoietin having one or more lysine residues deleted, or replaced with an amino acid other than lysne, and containing at least one polyalkylene glycol moiety covalently attached to at least one amino acid residue of the polypeptide.
Dependencies are inferred from the claim text.