Method of detecting AIDS virus infection

An immunoassay method for the detection of an AIDS virus related infection in a human host, which comprises:

• combining a body fluid from a human host with a monoclonal antibody that binds to an antigen on infected cells that is present only when said infection is present, and
• examining the combination for the presence of immune complexes comprising said antigen on said infected cells and said monoclonal antibody, the presence thereof indicating the presence of an AIDS virus infection, said antigen being capable of binding to one of the antibodies secreted by a cell line having the ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.

A method for determining the presence of an AIDS-related disease in a human host, which comprises detecting the presence of a cell-bound antigen associated with said disease in a sample of a body fluid from said human host, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.

An antibody capable of binding to an antigen produced as a result of a human HTLV-III infection, said antigen being bound to a lymphocyte, said antibody being selected from the group of antibodies secreted from hybrid continuous cell lines having ATCC accession numbers HB9101, HB9100, HB9114 and HB9102, respectively.

A diagnostic kit comprising in a packaged combination in separate containers

• a monoclonal antibody capable of binding to an antigen produced as a result of an infection by the viral causative agent of AIDS or a labeled derivative of said antibody, said antigen being bound to a lymphocyte as a result of said infection, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115, and
• a member of a signal producing system.

A composition of matter comprising a complex of:

• (a) a monoclonal antibody that binds HTLV-III gene product, said antibody being selected from the group of antibodies secreted from hybrid continuous cell lines having ATCC accession numbers HB9101, HB9100, and HB9102, respectively, and
• (b) said gene product.

A composition of matter comprising a complex of:

• (a) a monoclonal antibody capable of binding to a cell-bound antigen other than an HTLV-III gene product and produced as a result of an HTLV-III infection, said antibody being selected from the group of antibodies secreted from hybrid continuous cell lines having ATCC accession numbers HB9101, HB9100, and HB9102, respectively,
• (b) said antigen.

A monoclonal antibody having the identifying characteristics of binding to HTLV-III antigens of 23, 41, 55, 49, and 52 kilodaltons, respectively, said antigens being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.

A monoclonal antibody having the identifying characteristics of binding to HTLV-III antigens of 24, 28, 55, 65, and 49 kilodaltons, respectively, said antigens being capable of binding to one of the antibodies secreted by a cell line having ATCC accession number HB9100, HB9101, HB9102, HB9114, or HB9115.

A monoclonal antibody having the identifying characteristics of binding to HTLV-III antigens of 24;

• 41;
• and 55 kilodaltons, respectively, said antigens being capable of binding to one of the antibodies secreted by a cell line having ATCC accession number HB9100, HB9101, HB9102, HB9114, or HB9115.

A monoclonal antibody having the identifying characteristic of binding to HTLV-III antigens of 24, 39 and 65 kilodaltons, respectively, said antigens being capable of binding to one of the antibodies secreted by a cell line having ATCC accession number HB9100, HB9101, HB9102, HB9114, or HB9115.

A monoclonal antibody having the identifying characteristics of binding to an HTLV-III antigen of 55 kilodaltons, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.