Antibiotic F-0769, process for its production, and its use as a growth accelerating and feed efficiency increasing agent and as an antitumour agent

A process for producing antibiotic F-0769, comprising:

• culturing an antibiotic F-0769-producing microorganism belonging to the genus Streptomyces violaceusnigar F-0769 in a culture medium containing, as a carbon source, glucose, glycerol, sucrose, dextrin or starch, and as a nitrogen source, soybean meal, wheat embryo, peptone, meat extract, yeast extract, corn steep liquor or an ammonium salt, until a sufficient amount of said antibiotic has been imparted to said medium and isolating antibiotic F-0769 from th e culture product by a method which utilizes the difference in solubility between the antibiotic and impurities in the culture product or a method which utilizes the difference in absorption between the antibiotic and impurities in the culture product, or a combination of these, and
• obtaining antibiotic F-0769 characterized by the following properties:
• (1) physical appearance:
• white or light yellow powder;
• (2) melting point:
• 245.degree. to 250.degree. C.;
• (3) specific rotation:
• [.alpha.]25D=-37.5.degree. (c=1, methanol);
• (4) solubility in solvents;
• soluble in methanol, ethanol, acetone, chloroform, methylacetate and benzene;
• and insoluble in water and hexane;
• (5) elemental analysis (percent found):
• C=57.53, H=7.36, O=21.17, N=12.97;
• (6) ultraviolet absorption spectrum (as measured in methanol:
• as shown in FIG. 1, .lambda..sub.max (E1% 1cm)=213nm (466), 286nm (200));
• (7) infrared absorption spectrum (as measured by KBr method):
• as shown in FIG. 2;
• (8) nuclear magnetic resonance spectrum (as measured in CDCl.sub.3):
• .sup.1 H-NMR spectrum as shown in FIG. 3, and .sup.13 C-NMR spectrum as shown in FIG. 4);
• (9) distinction among basicity, acidity and neutrality:
• neutral substance;
• (10) amino acid analysis:
• as a result of hydrolysis with 6N hydrochloric acid at 110.degree. C. for 18 hours, detection of threonine, valine and leucine;
• (11) color reactions:
• positive in the iodine reaction and in the potassium permanganate reaction;
• and negative in the ninhydrin reaction and in the ferric chloride reaction;
• (12) thin layer of chromatography by means of silica gel, Art. 5715, manufactured by Merck Co.):
• ______________________________________ Solvent system Rf value ______________________________________ Ethyl acetate 0.16 Ethyl acetate-methanol (5:1) 0.33 Chloroform-methanol (10:1) 0.68 Ethyl acetate-acetone (1:1) 0.31 Acetone-benzene (5:1) 0.40 ______________________________________
• (13) molecular weight (as measured by the Rast method):
• about 745.