Compositions and methods for repair of cartilage and bone
OwnerSamuel Itayinventor
Life of this patent
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Abstract
A defect is provided in cartilage or bone, or both, to excise damaged or pathological tissue, and it is filled with an implant having capability for complete regeneration of the skeletal tissue as a chondrogenic or osteogenic phenotype. The implant comprises cells expressing a chondrocyte phenotype (80.times.10.sup.6 cells/ml) embedded in a biocompatible matrix having about 20% serum, which provides a permissive environment for maturation and transformation of the implant to a fully integrated state with the surrounding tissue. A portion of the implant may comprise a bone segment or a bone substitute.
Claims23
A method of regenerating skeletal tissue of a subject comprising the steps of:
- a culturing a proliferating cell population comprising autologous bone marrow stroma cells;
- b. manipulating them in suspension above soft agar at a concentration in excess of 2.times.10.sup.6 cell/ml of medium for at least five days;
- c. harvesting the cells;
- d. embedding the cells at a concentration in excess of 80.times.10.sup.6 cells/ml of a biological resorbable immobilization vehicle (BRIV) including in excess of 10% serum, 100 mg/ml fibrinogen, and 60 units/ml thrombin in an excess of 40mM CaC1.sub.2 and 2,000 units of aprotonin;
- e. removing a damaged part of the subject's skeletal tissue to leave a clean geometric shaped defect;
- and
- f. filling the defect with an implant at least including the combined cell concentration and BRIV.
The method as set forth in claim 11 above, wherein the BRIV includes 15-30% serum and 60 units/ml thrombin in an excess of 60mM CaC1.sub.2.
A method of regenerating skeletal tissue of a subject, comprising the steps of:
- a. culturing a proliferating cell population comprising autologous muscle fibroblast derived chrondocytes;
- b. manipulating the cells in suspension and culturing the cells above soft agar at a concentration in excess of 2.times.10.sup.6 cells/ml of medium for at least 3 days;
- c. harvesting the cells;
- d. embedding the cells at a concentration in excess of 80.times.10.sup.6 cells/ml of a biological resorbable immobilization vehicle (BRIV), the BRIV comprising in excess of 10% serum, 100 mg/ml fibrinogen, 60 units/ml thrombin in an excess of 40mM CaC1.sub.2, and 2000 units of aprotonin;
- e. removing a damaged part of the subject's skeletal tissue to leave a clean geometric shaped defect;
- and
- f. filling the defect with an implant at least including the combined cell concentration and BRIV.
A method of regenerating skeletal tissue in a person, comprising the steps of:
- a. obtaining a bone marrow sample from a donor;
- b. culturing a proliferative cell population comprising bone marrow osteogenic-chondrogenic progenitor cells;
- c. harvesting the cells;
- d. manipulating the cells in suspension above soft agar at a concentration in excess of 2.times.10.sup.6 cells/ml of medium for at least five days;
- e. embedding the cells at a concentration in excess of 80.times.10.sup.6 cells/ml of a biological resorbable immobilization vehicle (BRIV) including in excess of 10% serum, 100 mg/ml fibrinogen and 60 units/ml thrombin in 60 mM CaC1.sub.2 and 2000 units of aprotonin;
- f. removing a damaged part of the person's skeletal tissue to leave a clean geometric shaped defect;
- and
- g. filling the defect with an implant at least including the combined cell concentration and BRIV.
Dependencies are inferred from the claim text.