Process and composition for the purification of amphotericin B
AssigneeE. R. Squibb & Sons, Inc.
InventorsGerd W. Michel, Wilbur L. Bryan, Elizabeth Bryan
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Abstract
A unique four-solvent system comprising methanol, dimethylformamide, methylene chloride and water is employed to provide an improved process for the purification and crystallization of amphotericin B.
Claims10
An amphotericin B salt forming composition comprising amphotericin B, and an acid capable of forming a salt with said amphotericin B, in a solution comprising methanol, dimethylformamide, methylene chloride in an amount sufficient to enhance the crystallinity and purity of amphotericin B produced therefrom, and water.
A process for the purification and crystallization of amphotericin B which comprises
- a. forming a solution of amphotericin B in methanol, dimethylformamide and acid capable of forming a salt with said amphotericin B;
- b. separating amphotericin B extract from insolubles;
- c. combining said amphotericin B extract with a solvent mixture comprising methylene chloride in an amount sufficient to enhance the crystallinity and purity of amphotericin B produced therefrom, and water;
- d. adjusting the pH of the so-formed combination to between about 5 and 7;
- e. heating said slurry to expedite the formation of crystalline material;
- and
- f. recovering the crystalline amphotericin B.
Dependencies are inferred from the claim text.