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US 4,902,673 AGranted
Enhancing growth of bifidobacteria using soybean extract
AssigneeThe Calpis Food Industry Co., Ltd.
InventorsKunihiko Hayakawa, Teruhisa Masai, Yasuyuki Yoshida, Takanobu Shibuta, Hiroshi Miyazaki
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Abstract
A bidifobacteria growth promoter contained in soybeans is extracted from defatted soybeans with an aqueous solution of alcohol.
Claims5
A method promoting the growth of bifidobacteria, comprising the steps of culturing bifidobacteria in the presence of a growth promoting amount of a bifidobacteria growth promoter produced by steps including:
- mixing defatted soybean flakes with an aqueous solution of alcohol of a concentration of 20 to 80% by volume in the ratio 1:5 to 10 by weight;
- stirring the mixture;
- obtaining a supernatant alcohol-containing extract by removing insoluble materials from the mixture by filtration or centrifugation;
- and
- concentrating the supernatant by distillation under reduced pressure.
2 more elements
A method for promoting the growth of bifidobacteria in the digestive system of a subject, comprising the step of:
- orally administering a concentrated extract containing a bifidobacteria growth promoter to a patient in an amount effective to significantly promote the growth of bifidobacteria, said concentrated extract having been produced by:
- mixing defatted soybean flakes with an aqueous solution of alcohol of a concentration of 20 to 80% by volume in the ratio 1.5 to 10 by weight;
- stirring the mixture;
- obtaining a supernatant alcohol-containing extract by removing insoluble materials from the mixture by filtration or centrifugation;
- and
- concentrating the supernatant by distillation under reduced pressure.
3 more elements
A method of promoting the growth of bifidobacteria in a subject, comprising the steps of:
- orally administering a bifidobacteria growth promoter to a subject in an amount effective to significantly promote the growth of bifidobacteria, said bifidobacteria growth promoter having been produced by:
- mixing defatty soybean flakes with an aqueous solution of alcohol of a concentration of 20 to 80% by volume in the ratio of 1:5 to 10 by weight;
- stirring the mixture;
- obtaining a supernatant alcohol-containing extract by removing insoluble materials from the mixture by filtration or centrifugation;
- concentrating the supernatant by distillation under reduced pressure;
- and
- drying the resultant concentrated extract by spray-drying or lyophilizing.
4 more elements
Dependencies are inferred from the claim text.