Plasminogen activator
AssigneeSouth African Inventions Development Corporation
InventorEugene B. D. Dowdle
Life of this patent
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Abstract
For selectively and reversibly adsorbing tPA, pro-tPA or mixtures thereof, from an aqueous medium, for example, derived from a melanoma cell culture, the aqueous medium is intimately contacted with an affinity reagent comprising an immobilized Kunitz-type inhibitor, substantially as occurs in and is extractable from seeds of an Erythrina species, e.g., Erythrina latissima, having Erythrina species, the following combination of characteristics: (a) it is an inhibitor of the Kunitz-type; (b) it inhibits trypsin; (c) it inhibits plasmin; (d) it has no effect on urokinase; (e) it inhibits tPA; and (f) in its immobilized form, it is a selected adsorbent for tPA and its precursor pro-tPA, to thereby cause the tPA, pro-tPA or mixtures thereof to become selectively adsorbed on the affinity reagent.
Claims14
A process for selectively and reversibly adsorbing tPA (tissue plasminogen activator), pro-tPA or mixtures of both, from an aqueous medium containing such substance or substances in solution which comprises intimately contacting said aqueous medium with an affinity reagent comprising an immobilized Kunitz type inhibitor, derivable from an Erythrina species, and having the following combination of characteristics:
- (a) it is an inhibitor of the Kunitz type;
- (b) it inhibits trypsin;
- (c) it inhibits plasmin;
- (d) it has no effect on urokinase;
- (e) it inhibits tPA;
- and
- (f) in its immobilized form, it is a selective adsorbent for tPA and its precursor pro-tPA,
- to cause the tPA, pro-tPA or mixture of both to become selectively adsorbed on the affinity reagent.
A process applied to the purification of tPA, pro-tPA or mixtures of both which comprises intimately contacting an aqueous medium containing dissolved tPA, pro-tPA or a mixture of both with an affinity reagent comprising an immobilized Kunitz type inhibitor derivable from an Erythrina species, and having the following combination of characteristics:
- (a) it is an inhibitor of the Kunitz type;
- (b) it inhibits trypsin;
- (c) it inhibits plasmin;
- (d) it has no effect on urokinase;
- (e) it inhibits tPA;
- and
- (f) in its immobilized form, it is a selective adsorbent for tPA and its precursor pro-tPA,
- thereby selective adsorbing the tPA, pro-tPA or mixture of both from the medium;
- separating the affinity reagent loaded with tPA, pro-tPA or mixture of both from contact with said aqueous medium and non-adsorbed materials;
- and desorbing the tPA, pro-tPA or mixture of both from the affinity reagent and collecting the desorbed tPA, pro-tPA or mixture of both.
Dependencies are inferred from the claim text.