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US 4,902,621 AGranted
Test reagent for amylase determination
AssigneeAkzo N.V.
InventorPeter Witt
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Abstract
An aqueous stabilized enzyme preparation to be used for measuring the amount of .alpha.-amylase in a liquid sample like serum or urine containing a blocked and labeled oligosaccharide being the substrate for the .alpha.-amylase to be measured in the presence of exo-enzymes which aqueous preparation, preferably consisting of two separate solutions is stabilized by adding a buffer, a taurocholic acid derivative, an albumin, and a polyhydric alcohol.
Claims9
An aqueous stabilized enzyme preparation to be used for measuring the amount of .alpha.-amylase in a liquid sample comprising:
- (a). an oligosaccharide substrate for .alpha.-amylase comprising at least three glucose units, wherein the reducing-end glucose unit is coupled to a label cleavable by .alpha.- or .beta.-glucosidase, said label being one that exhibits a measurable change upon cleavage, and wherein the terminal glucose unit is blocked to inhibit cleavage by exo-enzymes,
- (b). at least two exo-enzymes being glucoamylase and at least one glucosidase selected from the group consisting of .alpha.- and .beta.-glucosidase, and
- (c). a stabilizing agent composition comprising:
- a buffer,
- a water-soluble metal salt of taurocholic acid,
- albumin and
- a polyhydric alcohol.
4 more elements
Dependencies are inferred from the claim text.