Production of hybridoma antibodies for interferon
AssigneeWadley Technologies, Inc.
InventorKurt F. Berg
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Abstract
A hybridoma continuous cell line, capable of producing monoclonal antibodies specific for all species of human interferon-alpha, is disclosed. A method for producing hybridomas and monoclonal antibodies with the required specificity is also disclosed. Methods of use of the disclosed compositions for purifying human interferon-alpha, screening blood in blood banks, and for producing specific polyclonal antibodies are claimed and detailed.
Claims2
A process for a producing a hybridoma cell capable of producing monoclonal antibodies specific for least one antigenic determinant common to all species of Human Interferon-alpha, comprising the steps of:
- (a) immunizing an animal with an immunogen comprising Human Interferon-alpha proteins admixed with an effective amount of sodium dodecyl sulfate to denature said proteins;
- and
- (b) thereafter removing at least one antibody-producing cell from said animal and fusing genetic material from said antibody-producing cell with an immortal cell to form said hybridoma cell.
Dependencies are inferred from the claim text.