Enzyme and process for producing the same
AssigneeHoffmann-La Roche Inc.
InventorsAkiko Fujiwara, Tatsuo Hoshino, Teruhide Sugisawa
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Abstract
The present invention relates to novel enzyme having L-sorbosone dehydrogenase activity and a process for producing the same. This L-Sorbosone dehydrogenase enzyme provided by the present invention catalyzes the oxidation of L-sorbosone to 2-keto-L-gulonic acid in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a coenzyme. 2-KGA is an important precursor for the production of vitamin C.
Claims7
The enzyme L-sorbosone dehydrogenase as a pure single protein.
The process of producing 2-keto-L-gulonic acid comprising oxidizing L-sorbosone in an inert solvent medium to form 2-keto-L-gulonic acid, said oxidation being carried out in said solvent medium containing a catalyst system containing the enzyme L-sorbosone dehydrogenase as a pure single protein and a coenzyme selected from the group consisting of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate and mixtures thereof.
Dependencies are inferred from the claim text.