Monoclonal antibody to human protein C
AssigneeTeijin Limited
InventorsKenji Wakabayashi, Yoshihiko Sumi, Yataro Ichikawa, Yoichi Sakata, Jun Mimuro, Nobuo Aoki
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Abstract
A monoclonal antibody which is specific to human protein C which has calcium bound at the gammacarboxyglutamic acid(Gla) domain and does not recognize human protein C which is not bound to calcium at the Gla domain. The monoclonal antibody is used in an immunoassay method for determining human protein C having a Gla domain and in a method for recovering human protein C having a Gal domain.
Claims4
A monoclonal antibody which is specific to human protein C which has calcium bound at the gamma-carboxyglutamic acid (Gla) domain and does not recognize human protein C which is not bound to calcium at the Gla domain.
A hybridoma, which produces a monoclonal antibody which is specific to human protein C which has calcium bound at the Gla domain and does not recognize human protein C which is not bound to calcium at the Gla domain.
A method of measuring human protein C having a gamma-carboxyglutamic acid (Gla) domain, which comprises:
- subjecting an aqueous mixture containing said human protein C having a Gla domain, in the presence of a calcium ion, to an antigen-antibody reaction using a monoclonal antibody specific to a complex of human protein C which has calcium bound at the Gla domain, and
- subjecting the resulting mixture to a measurement of the amount of the antigen-antibody reaction product as a measure of said human protein C.
A method of recovering human protein C having a gamma-carboxyglutamic acid (Gla) domain, which comprises:
- bringing an aqueous mixture containing human protein C having a Gla domain in the presence of a calcium ion into contact with a fixed monoclonal antibody comprising an insoluble carrier and fixed thereto a monoclonal antibody specific to a complex of human protein C binding calcium at the Gla domain, whereby the human protein C is captured by the fixed monoclonal antibody in the form in which the calcium ion is bound to the Gla domain,
- separating the captured human protein C from the fixed monoclonal antibody,
- separating step being carried by contacting the monoclonal antibody capturing the human protein C having the calcuim ion bound to its Gla domain with an aqueous medium substantally free from a calcium ion, whereby the human protein C is separated from the fixed monoclonal antibody in a form not bing the calcium ion in the Gla domain, and
- recovering the separated human protein C.
Dependencies are inferred from the claim text.