Apparatus for simultaneous visualization and measurement of fluorescence from fluorescent dye-treated cell preparations and solutions
AssigneeUniversity of Utah
InventorsGeorge A. Peeters, William H. Barry
Life of this patent
Estimating the term…
Abstract
An apparatus for measuring multiple wavelengths of fluorescence emitted from fluorescent dye-treated cells, tissue preparations, or solutions which is comprised of a custom designed optical module into which is integrally fitted a source of long wavelength red light, a specimen stage, an epifluorescence system, a high intensity ultraviolet light source for excitation, an optical subassembly which permits simultaneous fluorescence detection and real time visualization of the subject under study. A standard microscope may be used in place of the custom designed optical module for integration of the component systems.
Claims9
An apparatus for measuring fluorescence at multiple wavelengths emitted from fluorescent dye-treated cell or tissue preparations or solutions, comprising in combination:
- an optical housing unit means with at least two access ports therein;
- An epifluorescence system integrally connected to said optical housing unit through one of said access ports therein;
- at least one source of high intensity ultraviolet light for excitation connected to said optical housing unit through one of said access ports therein;
- an optical subassembly means with at least two access ports therein, said optical subassembly being connected to said optical housing unit through one of said access ports therein;
- means for fluorescence detection based within and connected to said optical subassembly through one of said access ports therein;
- means for video monitoring connected to said optical subassembly means through one of said access ports therein;
- and,
- at least one source of illumination whose wavelength is longer than the excitation wavelength.
An apparatus for measuring fluorescence at multiple wavelengths emitted from fluorescent dye-treated cell of tissue preparations or solutions, comprising in combination:
- an epifluorescence system;
- at least one source of high intensity ultraviolet light for excitation;
- an optical subassembly means with at least two access ports therein;
- means for fluorescence detection housed within and connected to said optical subassembly through one of said access ports therein;
- means for video monitoring connected to said optical subassembly through one of said access ports therein;
- and
- at least one source of illumination whose wavelength is longer than the excitation wavelength.
Dependencies are inferred from the claim text.