New hydrolase substrates
AssigneeBoehringer Mannheim GmbH
InventorsHerbert von der Eltz, Hans-Joachim Guder, Klaus Muhlegger
Life of this patent
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Abstract
Reagents for the determination of hydrolase comprises compounds of the formula ##STR1## wherein Z is an organic or inorganic acid residue or a sugar residue, A is an organic or inorganic acid residue, and B is an organic acid residue. Each of R.sup.2 and R.sup.5, is hydrogen, halogen or lower alkyl. Each of R.sup.1, R.sup.3, R.sup.4 and R.sup.6, is hydrogen, halogen, cyano, lower alkyl, lower alkoxy, carboxyl, lower alkoxycarbonyl, carboxy lower alkyl or lower alkoxycarbonyl lower alkyl, or carboxamido groups optional substituted once or twice, or a radical of the formula --COO--(CH.sub.2 CH.sub.2 O).sub.n --R.sup.7 in which R.sup.7 is hydrogen or lower alkyl and n is a number from 1 to 4. Additionally, R.sup.6 can be sulpho or nitro.
Claims15
A compound of the formula ##STR5## wherein one of Z and A is an orthophosphoric or a pyrophosphoric acid residue or a sulphuric acid residue;
- the other of Z and A is an orthophophosphoric, pyrophosphoric or a sulphuric acid residue;
- a saturated or unsaturated (C.sub.1 -C.sub.20)-alkylcarbonyl;
- an amino acid or an oligopeptide residue bound by their carboxylic acid ends;
- or, if Z is an orthophosphoric, pyrophosphoric or sulphuric acid residue, A may also be (C.sub.1 -C.sub.7)-alkylcarbonyl, optionally unsaturated, which is substituted one or more times by hydroxyl, carboxyl or an ammonium group;
- B is a (C.sub.1 -C.sub.7)-alkylcarbonyl optionally substituted one or more times by hydroxyl, carboxyl or an ammonium group;
- each of R.sup.2 and R.sup.5 is hydrogen, halogen or (C.sub.1 -C.sub.5)-alkyl;
- each of R.sup.1, R.sup.3, R.sup.4 and R.sup.6 is hydrogen;
- halogen, cyano;
- (C.sub.1 -C.sub.5)-alkyl;
- (C.sub.1 -C.sub.5)-alkoxy;
- carboxyl;
- (C.sub.1 -C.sub.5)-alkoxycarbonyl;
- carboxy-(C.sub.1 -C.sub.5)-alkyl;
- (C.sub.1 -C.sub.5)-alkoxycarbonyl-(C.sub.1 -C.sub.5)-alkyl;
- carboxamido optionally substituted once or twice by (C.sub.1 -C.sub.5)-alkyl, (C.sub.1 -C.sub.5)-alkoxy-(C.sub.1 -C.sub.5)-alkyl, carboxy-(C.sub.1 -C.sub.5)-alkyl or (C.sub.1 -C.sub.5)-alkoxycarbonyl-(C.sub.1 -C.sub.5)-alkyl), or in the case of disubstitution, the two substitutents can be joined to form a ring which can be interrupted by oxygen, nitrogen and sulphur atoms;
- or a radical of the formula
- --COO--(CH.sub.2 CH.sub.2 O).sub.n --R.sup.7
- in which R.sup.7 is a hydrogen or (C.sub.1 -C.sub.5)-alkyl, and n is a whole number of from 1 to 4;
- and
- R.sup.6 can also be sulpho or nitro.
Dependencies are inferred from the claim text.